Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMKQFEIAAIPGDGVGKEVVAAAEKVLHTAAEVHGGLSFSFTAFPW-SCDYYLEHGKMMPEDGIHTLTQFEAVFLGAVGNPKLVPDHISLWGLLLKIRRELELSINMRPAKQMAGITSPLLHPN--DFDFVVIRENSEGEYSEVGGRIHRG-DDEIAIQNAVFTRKATERVMRFAFELAKKR-RSHVTSATKSNGIYHAMPFWDEVFQQTAADYSGIETSSQHIDALAAFFVTRPETFDVIVASNLFGDILTDISSSLMGSIGIAPSANINPSGKYPSMFEPVHGSAPDIAGQGLANPIGQIWTAKLMLDHFGE-----EELGAKILDVMEQVTADGIKTRDIGGQSTTAEVTDEICSRLRKL 3FMX Chain:X ((5-363)) ----SFRIAAIPGDGIGLEVLPEGIRVLEAAALKHG-LALEFDTFEWASCDYYLQHGKMMPDDWAEQLKQYDAIYFGAVGWPDKVPDHISLWGSLLKFRREFDQYVNIRPVRLFPGVPCALANRKVGDIDFVVVRENTEGEYSSLGGIMFENTENEIVIQESIFTRRGVDRILKYAFDLAEKRERKHVTSATKSNGMAISMPYWDKRTEAMAAHYPHVSWDKQHIDILCARFVLQPERFDVVVASNLFGDILSDLGPACAGTIGIAPSANLNPERNFPSLFEPVHGSAPDIFGKNIANPIAMIWSGALMLEFLGQGDERYQRAHDDMLNAIERVIADGSVTPDMGGTLSTQQVGAAISDTLARL

General information: TITO was launched using: RESULT: Template: 3FMX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1980 -177090 -89.44 -507.42 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain X : 0.86 3D Compatibility (PKB) : -89.44 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_3FMX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FMX-query.scw PDB file : Tito_Scwrl_3FMX.pdb: