Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIAGYIMAAENQTLNQWTINQLGITRGDSILEVGFGPGYCMQQMLKREKDVHLHGIDVSEAMLKLAARRVKPKG----VRLIQGSIETFPLPASFYDKVISVNNYTIWNDQTKGIKQIYRALKPGGKAAITMQPREADASPEKTKSFGRQMIADFKAAGFEDIDIQFKNIKPELSVCATAKKPAT 3DLC Chain:A ((29-146)) ----YPIIAEN------IINRFGITAG-TCIDIGSGPG-ALSIALAKQSDFSIRALDFSKHMNEIALKNIADANLNDRIQIVQGDVHNIPIEDNYADLIVSRGSVFFWEDVATAFREIYRILKSGGKTYI-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3DLC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 577 -73486 -127.36 -644.61 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -127.36 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.685

(partial model without unconserved sides chains): PDB file : Tito_3DLC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DLC-query.scw PDB file : Tito_Scwrl_3DLC.pdb: