Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHVITTQVLFIFCFLLLIHSIETLAYATRLSGARVGFIASALSLFNVMVIVSRMSNMVQQPFTGHLIDDAGKNALAIVGEQFRFLIFGSTVGTILGIILLPSFVALFSRAIIHLAGGGGSVFQVFRKGFSKQGFKNALSYLRLPSISYVKGFHMRLIPKRLFVINMLITSIYTIGVLSALYAGLLAPERSTTAVMASGLINGIATMLLAIFVDPKVSVLADDVAKGKRSYIYLKWTSVTMVTSRVAGTLLAQLMFIPGAYYIAWLTKWF 2ZJI Chain:A ((340-368)) ---------------------------------------------------------------------------------YKFAISQSSCGTVMGAVIMEGFYVVFDRA---------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ZJI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 18 -6074 -337.42 -209.43 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.42 3D Compatibility (PKB) : -337.42 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.42 QMean score : 0.121

(partial model without unconserved sides chains): PDB file : Tito_2ZJI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZJI-query.scw PDB file : Tito_Scwrl_2ZJI.pdb: