Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKTNDFMEIMKGRRSIRNYDPAVKISKEEMTEILEEATTAPSSVNAQPWRFLVIDSPEGKEKLAPLASFNQTQVTTSSAVIAVFADMNNADYLEEIYSKAVELGYMPQEVKDRQIAALTAHFEKLPAQVNRETILIDGGLVSMQLMLTARAHGYDTNPIGGYDKENIAETFGLDKERYVPVMLLSIGKAADEGYASYRLPIDTIAEWK 3GE6 Chain:A ((9-212)) -----DFMEIVKGRRSIRNYDTNVKISKEEMTQILEEATLAPSSVNMQPWRFLVIDSEEGKATLAPLAKFNQVQVETSSAVIAVFGDMKAIDQLENIYDTAVEKGLMPQEVRDRQVPAIQGMYENVPASALKDSILIDSGLVSMQLMLVARAHGYDTNPIGGYEKDQIAEAFGMEKDRYVPVMLLSIGKAVDAGYPSVRLPINDIADWK

General information: TITO was launched using: RESULT: Template: 3GE6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 842 -98837 -117.38 -484.49 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.92 3D Compatibility (PKB) : -117.38 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_3GE6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GE6-query.scw PDB file : Tito_Scwrl_3GE6.pdb: