Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNFILNENKQLSLAIEDENIENFYIDGTDLVRKIIRRSGSGVTSRVPVLSTQDLENKNLHELYDESWLRMKNRPNTELTTESINIADLFSGCGGLSLGVWEACRALGINPRFSFACDLNEAALSVYEKNFSPDFSLNESIEKHINGELGAPLTVEEQRIKDKVKK---IDFILAGPPCQGHSDLNNHTRRKDPRNALLMRVSRVIELFQPSSVLVENVPGIIHDKSGSFKEFKNHLKTQGY-YFDEIVLNAEKLGVSQARRRYFIFASKTPVSSLNQINEFYSTNSRPISWAISDLVENVGDDIFNTASEHSLENKRRIEYLFENNLFE------------------LPNSERPDC-----HRLKPHSYKSV-YG---------RMYWDRPAPTITRGFGSTGQGRFVHSLLKRTITPHEAARIQFFPDFFNFGDLRRRQYQDVIGNAV-PSKLSYLLALHQLR 3G7U Chain:A ((2-360)) ----------------------------------------------------------------------------------SLNVIDLFSGVGGLSLG---AARA-GFDVKM--AVEIDQHAINTHAINFPRSLHVQEDVSL-LNAEI----------IKGFFKNDMPIDGIIGGPPCQGFS--------DDSRNQLYMHFYRLVSELQPLFFLAENVPGIMQEKYSGIRNKAFNLVSGDYDILDPIKVKASDYGAPTIRTRYFFIGVKKSLKLDISDEVFMPKMIDPVT--VKDALYGL-PDIIDANWQSDSESWRTIKKDRKGGFYEKLWGQIPRNVGDTESIAKLKNNIISGCTGTLHSKIVQERYASLSFGETDKISRSTRLDPNGFCPTL---------VRPIHPYHPRVITPREAARLQGFPDWFRFHVTKWHSFRQ-IGNSVSPIVAEYIL------

General information: TITO was launched using: RESULT: Template: 3G7U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1610 -136401 -84.72 -453.16 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -84.72 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.311

(partial model without unconserved sides chains): PDB file : Tito_3G7U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G7U-query.scw PDB file : Tito_Scwrl_3G7U.pdb: