Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDLYQLIGEKLNDIIPGEWTKIYLYA-EVLDDSTMVLFHFRTPENNQIIYSQDIPSHYNVSKDIFKTLLRELRELFEELRTEHRNNNDDVWTNLTLTLDRSGEFQLDYNYDDILASELDGYERIAIWEYKNLGILPEDEDDKEFVISYLGL 2IA1 Chain:A ((6-160)) IESYYQEIAQLIIDMIPEEWAEVRFYAQEDHDGWKIFFFHYLSASSDEWTKDIDIRDVIKVPQDEFMEKYNELSFCISDFRKDYAEAFGEPWMSFQMTFYASGKFNIDFYYD---KNPFDTFLTRLAWQYEHFGTIP-DSFYKETLNEYL--

General information: TITO was launched using: RESULT: Template: 2IA1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 536 -81818 -152.65 -564.26 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -152.65 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.652

(partial model without unconserved sides chains): PDB file : Tito_2IA1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IA1-query.scw PDB file : Tito_Scwrl_2IA1.pdb: