Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METTKLGNLKLYGAGHAAGGAYHNVSIKGEGIVGEGLSAVGCRIYGTGLFLGKAETERLRVLGESECKGDLTAGKINIYGTMKVSGSLQFDRFNLKGQTEIGGNMTGESCDVKGKLSVIGDC----ETEMFHVTGCVDVSGLLNSGEIKLGLSHD--ISHVQEIGGTTIT---VKRRASFFSRKKGKLIADVIEGDRVYLENTEAAVVRGKEVIIGPGCSIGTIEYEYKCECDPHSQIKEKTKL 2PIG Chain:A ((86-198)) -----------------------------------------------------------------------------LYNNVRIGDNVWIDRADISDGARISDNVTIQSSSVREECAIYGDARVLNQSEILA--------AQILQIYDRATVNHSRIVHQVQLYGNATITHAFIEHRAEVFDFA-------LIEGDK-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2PIG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 533 -54383 -102.03 -566.49 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -102.03 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.269

(partial model without unconserved sides chains): PDB file : Tito_2PIG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PIG-query.scw PDB file : Tito_Scwrl_2PIG.pdb: