Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQKAVILDEQAIRRALTRIAHEMIERNKGMNNCILVGIKTRGIYLAKRLAERIEQIEGNPVTVGEIDITLYRDDLSKKTSNDEPLVKGADIPVDITDQKVILVDDVLYTGRTVRAGMDALVDVGRPSSIQLAVLVDRGHRELPIRADYIGKNIPTSKSEKVMVQLDEVDQNDLVAIYENE 1A4X Chain:A ((4-180)) ---KAVILDEQAIRRALTRIAHEMIERNKGMNNCILVGIKTRGIYLAKRLAERIEQIEGNPVTVGEIDITLYR----------EPLVKGADIPVDITDQKVILVDDVLYTGRTVRAGMDALVDVGRPSSIQLAVLVDRGHRELPIRADYIGKNIPTSKSEKVMVQLDEVDQNDLVAIYEN-

General information: TITO was launched using: RESULT: Template: 1A4X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 935 -126774 -135.59 -759.13 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.98 3D Compatibility (PKB) : -135.59 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.98 QMean score : 0.648

(partial model without unconserved sides chains): PDB file : Tito_1A4X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1A4X-query.scw PDB file : Tito_Scwrl_1A4X.pdb: