Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEMDITQYLSTQGPFAVLFCWLLFYVMKTSKERESKLYNQID------SQNEVLGKFSEKYDVVIEKLDKIEQNFK 2AAA Chain:A ((256-297)) --------------------WQLLYAFESSSGSISNLYNMIKSVASDCSDPTLLGNFIENHD--------------

General information: TITO was launched using: RESULT: Template: 2AAA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 47 -1843 -39.20 -51.18 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -39.20 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.324

(partial model without unconserved sides chains): PDB file : Tito_2AAA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AAA-query.scw PDB file : Tito_Scwrl_2AAA.pdb: