Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEKTFIMVKPDGVQRQLIGDILSRFERKGLQLAGAKLMRVTEQMAEKHYAEHQGKPFFGELVEFITSGPVFAMVWEGENVIEVTRQLIGKTNPKEALPGTIRGDYGMFVGKNIIHGSDSLESAEREINIFFKNEELVSYQQLMAGWIY 2VU5 Chain:A ((1-148)) -MEKTFLMVKPDGVQRAFIGEIVARFEKKGFQLVGAKLMQVTPEIAGQHYAEHEEKPFFGELVDFITSGPVFAMVWQGEGVVDTARNMMGKTRPHEAAPGTIRGDFGVTVAKNIIHGSDSLESAEREIGIFFKEEELVDYSKLMNEWIY

General information: TITO was launched using: RESULT: Template: 2VU5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 696 -73898 -106.18 -499.31 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.95 3D Compatibility (PKB) : -106.18 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_2VU5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VU5-query.scw PDB file : Tito_Scwrl_2VU5.pdb: