Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKNITLTILLTNLFIAFLGIGLVIPVTPTIMNELHLSGTA-VGYMVACFAITQLIVSPIAGRWVDRFGRKIMIVIGLLFFSVSEFLFGIGKTVEMLFISRMLGGISAAFIMPGVTAFIADITTIKTRPKALGYMSAAISTGF----IIGPGIGGFLAEVHSRLPFFFAAAFALLAAILSILTLREPERNPENQEIKGQKTGFKRIFAP--MYFIAFLIILISSFGLASFESLFALFVDHKFGFTASD-----IAIMITGGAIVGAITQVVLFDRFTRWFGEIHLIRYSLILSTSLVFLLTTVHSYVAILLVTVTV-FVGFDLMRPAVTTYLSKIA-GNEQGFAGGMNSMFTSIGNVFGPIIGGMLFDI-DVNYPFYFATVTLAIGIALTIAWKAPAHLKAST 3WDO Chain:A ((26-396)) -------------------LGMFMVLPVLTTYGMALQGASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIWGIILGRALQGSGA--IAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITHKLG-LHAL--FWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLNFGIMCLHMLLMSTFVALPGQLADA--GFPAAEHWKVYLATMLIA---FGSVVPFIIYAEVKRKMKQVFVFCVGLIVVAEIV--LWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAGYKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPPYVSS--

General information: TITO was launched using: RESULT: Template: 3WDO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1745 -198686 -113.86 -558.11 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -113.86 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.308

(partial model without unconserved sides chains): PDB file : Tito_3WDO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WDO-query.scw PDB file : Tito_Scwrl_3WDO.pdb: