Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALSPEIEFEDIEDDSEVIETSQTYKIDFENGRITNEIITG-----LEAIKQFV--------YLSLHTERYAYSVYSHDIGNELQDVLADNETTDAYKKMEIPRLIEEALIYDDRISAVTDFEIEKQGESFRVSFTVETDEGTLEIEEVLGEDV 2QZU Chain:A ((374-450)) ----------------EIPETVQTFDL-------SNEVLTGKNKKDLVQPYYFVKFDNHATGYRGLRTDRYTYAVHATD--GKIDNVILFDRTNDPHEMNNI----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2QZU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 139 1785 12.84 27.89 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 12.84 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.231

(partial model without unconserved sides chains): PDB file : Tito_2QZU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QZU-query.scw PDB file : Tito_Scwrl_2QZU.pdb: