Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRGLDQFIQSLDRASRGGLKRKYEQWLESMGF---EFLDIIQDEIIRTKT-----VDTRRLLNSFQKGDQDNIFSMTEGSLKLDVGTNLDYASYVNDGHFTIDPSKNQDRRWV----PGRWKGDRFEYDPAEKNSGMLLKFRW---VDGSGFWDNAMAIFQLMFERSLERKLQQWIYEEF 1BCM Chain:A ((264-391)) --------------------------QWINGDGYLHNVFVRWFNGDVIRPKTWFWQDVKTRKIL-GWRCDVSENI-----DSIRLSFMDVVTRYGIPEDFHITIDNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKG-WGQAKPV---------------------

General information: TITO was launched using: RESULT: Template: 1BCM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 420 34857 82.99 308.47 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 82.99 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.110

(partial model without unconserved sides chains): PDB file : Tito_1BCM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BCM-query.scw PDB file : Tito_Scwrl_1BCM.pdb: