Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEFMKKFSLTKPIIQAPMAGGITKPRLASAVSNQGALGSLASGYLTPDLLEQQIKEIFELTDAPFQINVFVPLGLEMPPKDQIKKWKENI-PLANQVNQFTSVQEE--WDDFYQK---IDLILKYKVKACSFTFDLPPEDAVKELKTAGCCLIGTASTVEEALLMEERGMDIVVLQGSEAGGHRGAFLPSKGESAVGLMALIPQAADALSVPVIAAGGMIDHRGVKAALTLGAQGVQIGSAFLICHESNAHPVHKQKILEANEADTKLTTLFSGKEARGIVNKWMEENEQFETQTLP-YPYQNTLTKAMRQKASLQNNHDQMSLWAGQGIRSLTEEISVKQLLNQLCQEDIKI 4QIS Chain:A ((9-353)) --RFTRLLGIQQPIIQAPMLG-VSTPALAAAVSNAGGLGSIAITGSAAEKGRALIREVRGLTDKPFNVNLFCHRPGQADPARE-RAWLDYLKPLFAEFGAEPPVRLKNIYLSFLEDPTLLPMLLEERPAAVSFHFGAPPRDQVRALQAVGIRVLVCATTPEEAALVEAAGADAVVAQGIEAGGHRGVFEPERGDAAIGTLALVRLLAARGSLPVVAAGGIMDGRGIRAALELGASAVQMGTAFVLCPESSANAAYREALKGPRAARTALTVTMSGRSARGLPNRMFFDAAAPGVPPLPDYPFVYDATKALQTAALARGNHD-FAAQWAGQGAALARELPAAELLRTLVEE----

General information: TITO was launched using: RESULT: Template: 4QIS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2097 11869 5.66 35.11 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 5.66 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_4QIS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QIS-query.scw PDB file : Tito_Scwrl_4QIS.pdb: