Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRLIQVMIMFTLLLTMAFSADAADSSYYDEDYRPQYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLQWGPMHWGHAVSKDLVTWEHLPVALYP-----DEKGTIFSGSAVVDKNNTSGFQTGKEKPLVAIYTQ-------------DREGHQVQSIAYSNDKGRTWTKY-AGNPVIPNPGK------KDFRDPKVFWYEKEKKWVMV--LAAGDRILIYTSKNLKQWTYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKKWVMQVSVGNGAVSG--GSGMQYFVGDFDGTHFKNEN----PPNKVL-WTDYGRDFYAAVSWSDIPSTDSRRLWLGWMSNWQYANDVPTSPWRSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTSKKWKNLTISPASHNVLAGQSGDAYEINAEFKVSPGSAAEFGFKVRTGEN--QFTKVGYDRRNAKLFVDRSESGNDTFNPAFNTGKETAPLKP-VNGKVKLRIFVDRSSVEVFGNDGKQVITDIILPDRSSKGLELYAANGGVKVKSLTIHPLKKVWGTTPFMSNMTGWTTVNGTWADTIEGKQGRSDGDSFILSSASGSDFTYESDITIKDGNGRGAGALMFRSDKDAKNGYLANVDAKHDLVKFFKFENGAASVIAEYKTPIDVNKKYHLKTEAEGDRFKIYLDDRLVIDAHDSVFSEGQFGLNVWDATAVFQNVTKES 1Y9M Chain:A ((3-517)) -----------------------------YDQPYRGQYHFSPQKNWMNDPNGLLYHNGTYHLFFQYNPGGIEWGNISWGHAISEDLTHWEEKPVALLARGFGSDVTEMYFSGSAVADVNNTSGFGKDGKTPLVAMYTSYYPVAQTLPSGQTVQEDQQSQSIAYSLDDGLTWTTYDAANPVIPNPPSPYEAEYQNFRDPFVFWHDESQKWVVVTSIAELHKLAIYTSDNLKDWKLVSEFGP-YNAQGGVWECPGLVKLPLDSG-NSTKWVITSGLNPGGPPGTVGSGTQYFVGEFDGTTFTPDADTVYPGNSTANWMDWGPDFYAAAGYNGLSLND--HVHIGWMNNWQYGANIPTYPWRSAMAIPRHMALKTIGSKATLVQQPQEAWSSISNKRPIYSRTFKTLSEGSTNTTTTGETFKVDLSFSAK-SKASTFAIALRASANFTEQTLVGYDFAKQQIFLDRTHSGDVSFDETFASVYH-GPLTPDSTGVVKLSIFVDRSSVEVFGGQGETTLTAQIFPSSDAVHARLASTGGTTEDVRADIYKIASTW--------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1Y9M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2913 133874 45.96 280.07 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 45.96 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_1Y9M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y9M-query.scw PDB file : Tito_Scwrl_1Y9M.pdb: