Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFLQPLLAKKIIAEVKKMYEREVIIVNTDGLIMAGTNDERVGQFHEGALICAKERRSVIITKEDETRLKGVKAGINLPVFFDHDVIAVFGLTGEPAEIQPFGELLRKMTELFIKESRHLEQSQWRERMLESFMIDWLQLKEWSPSFLEKAQLLGVDLSSRRQMILIQGYEWSPHDIEQMARSWKSSYPADLFIRWGNERILINHEVPQHEQRDRLLRKILHICSFANTASSQYTAAGAGRAVASSSLTDSYEQAEKALAVSLKRKTPIFEEDLKLDMCLTEISPGTRNEFPQRVLGKALEHQELMNTIRTFFHHDLSLKQTAEDMHIHINTLRYRLAKAEQLTGLRFDRTEDVVTMYVALYFLDQDTK 3ONQ Chain:A ((201-254)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TVSTFLKYGSSLENTAKELNVHPNTVRYRLKRAAETTGWDATDPRDAYVLTTAL--------

General information: TITO was launched using: RESULT: Template: 3ONQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 184 -22288 -121.13 -412.74 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -121.13 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_3ONQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ONQ-query.scw PDB file : Tito_Scwrl_3ONQ.pdb: