Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILFAVSECTPFVKSGGLADVAGALPKALARLGNEVAVMLPKY--SQIPEPWKKRMKKQAECTVAVGWRQQYCGIEHMAENDVNYYFIDNEYYFNRDSLYGHY-----DDGERFAFFSRAVLEAAKVVNV------QADIVHTHDWHTAMVNYLLKEEYRKHPFYERMKSVLTIHNLQFQGIFPPDVTHDLL-------GLEMDHFHYER---LECNGFVNFMKAGIIAADHVTTVSPTYRNEIMTPYYGEQLEQVLQYREDDVTGILNGIDDTFYQPKSDPYIEAQYDSGDLACKLENKTKLQQRMGLPEKNDIPLISMVTRLTKQKGLDLVRRIMHELLEEQDIQLVVLGTGEREFEDYFRYAEFAFHEKCRAYIGFDEPLAHQIYAGSDMFLMPSKFEPCGLGQLIALQYGAIPIVRETGGLYDTVRAY-----QEEEGTGNGFTFSAFNAHDLKFTIERALS-FYCQQDVWKSIVKTAMNADYSWGKSAKEYQRIFEQVTRSGRDVLE 4HLN Chain:A ((126-623)) -SIVFVTGEAAP--------DVCGSLPIALAARGHRVMVVMPRYLNGTSDKNYAKALYTGKHIKIPCFGGSHEVTFFHEYRDNVDWVFVDHPSYHRP------NFGAFGDNQFRYTLLCYAACEAPLILELGGYIYGQSCMFVVNDWHASLVPVLLAAKYRPYGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHALDKGEAVNFLKGAVVTADRIVTVSQGYSWEVTTAEGGQGLNELLSSRKSVLNGIVNGIDINDWNPTTDKCLPHHYSVDDLSGKAKCKAELQRELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPDLMRE-DVQFVMLGSGDPVFEGWMRSTESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTGGLRDTVETFNPFGAKGEEGT--GWAFSPLTVEKMLWALRTAISTFREHKPSWEGLMKRGMTKDHTWDHAAEQYEQIFE-----------

General information: TITO was launched using: RESULT: Template: 4HLN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2453 -25618 -10.44 -56.30 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -10.44 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_4HLN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HLN-query.scw PDB file : Tito_Scwrl_4HLN.pdb: