Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTVREAKLEDIKDIAKVHVDSWRTTYHEIIPIDYLNSLNYKEFEDKWKSR----SLKG-VFVAQDEKGSVFGFASFGPIRSEQ-EGYDGELYAIYLLEERQRQGAGRALLAKGAEFLLQHGFSSMFVWVIEQNP-SIIFYQAYSPERVAEDNFEIAGVRLKEVGLGWPDLSALKTLLNR 1WK4 Chain:A ((1-173)) -VRIRRAGLEDLPGVARVLVDTWRATYRGVVPEAFLEGLSYEGQAERWAQRLKTPTWPGRLFVAESESGEVVGFAAFGPDRASGFPGYTAELWAIYVLPTWQRKGLGRALFHEGARLLQAEGYGRMLVWVLKENPKGRGFYEHLGGVLLGEREIELGGAKLWEVAYGF-DLGGHK-----

General information: TITO was launched using: RESULT: Template: 1WK4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 768 4402 5.73 26.52 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : 5.73 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_1WK4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WK4-query.scw PDB file : Tito_Scwrl_1WK4.pdb: