Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLVLIGGGYGNMRVLHRLLPNQLPDDVSITLIDRNPYHCLKTEYYALAAGTISDHHIRVSFPE---HPRLDVQYGDITSIDIVQKQVLFQDREPISYDDAIIGLGCEDKYHNVPGAPEFTYSIQTIDQSRETYQKLNNLSAN----------ATVAIVGAGLSGVELASELRESRDDL-----------NIILFDRGNLILSSFPERLSKYVQKWFEEHGVRIINRANITKVE-EGVVYNHD---DPISADAIVWTAGIQPNKVVRDLDVEKDAQGRIVLTPHHNLPGDEHLYVVGDCASL-------PHAPSAQLAEAQAEQIVQILQKRWNGEALPESMPQFKLKGVLGSLGKKAGFGLVADRPLIGRVPRMLKSGLLWMYKHHNG 4NWZ Chain:A ((6-376)) ---IVILGAGYGGIVAALGLQKRLNYNEADITLVNKNDYHYITTELHQPAAGTMHHDQARVGIKELIDEKKIKFVKDTVVAIDREQQKVTLQNGE-LHYDYLVVGLGSEPETFGIEGLREHAFSINSINSVRIIRQHIEYQFAKFAAEPERTDYLTIVVGGAGFTGIEFVGELADRMPELCAEYDVDPKLVRIINVEAAPTVLPGFDPALVNYAMDVLGGKGVEFKIGTPIKRCTPEGVVIEVDGEEEEIKAATVVWTGGVRGNSIVEKSGFET-MRGRIKVDPYLRAPGHENIFIVGDCALIINEENNRPYPPTAQIAIQHGENVAANLAALIRGGSMTPFKPH--IRGTVASLGRNDAIGIVGGRKVYGHAASWLK------------

General information: TITO was launched using: RESULT: Template: 4NWZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1831 -35047 -19.14 -104.31 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -19.14 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_4NWZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NWZ-query.scw PDB file : Tito_Scwrl_4NWZ.pdb: