Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILIQNAEFELVHNFKDGFNEEAFKARYSDILNKYDYIVGDWGYGQLRLKGFFDDQNQKATFETKISTLDEYIYEYCNFGCAYFVLKRIRK 2KL5 Chain:A ((12-102)) MILIQNAEFELVHNFKDGFNEEAFKARYSDILNKYDYIVGDWGYGQLRLKGFFDDQNQKATFETKISTLDEYIYEYCNFGCAYFVLKRIRK

General information: TITO was launched using: RESULT: Template: 2KL5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 371 -56352 -151.89 -619.25 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 1.00 3D Compatibility (PKB) : -151.89 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_2KL5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KL5-query.scw PDB file : Tito_Scwrl_2KL5.pdb: