Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCAVKTG-LPTELDLQEPERVADSVALMNLKHAVITAVARDDQKDGGAGIFAETVRAIRRKSPFTTIEVLPSDMG------GNYDNLKTLMDTRPDILNHNIETVRRLTPRVRARATYDRSLEFLRRAKEMQPDIPTKSSIMIGLGETKEEIIEVMDDLLANNVDIMAIGQYLQPTKKHLKVQKYYHPDEFAELKEIAMQKGFSHCEAGPLVRSSYHADEQVNEASKKRQAQA 4U0O Chain:B ((8-288)) ----------PSWLR--------ISTVQRLVRQYGIHTICEEGRCPNRGECYG-QKTATFLLLGPTCTRACAFCQVEKGHAPAAVDPEEPTKIAAAVATLGLRYVVLTSVARDDLPDQGAGQFVATMAAIRQRCPGTEIEVLSPDFRMDRGRLSQRDCIAQIVAAQPACYNHNLETVRRLQGPVRRGATYESSLRVLATVKELNPDIPTKSGLMLGLGETEAEIIETLKDLRRVGCDRLTLGQYLPPSLSHLPVVKYWTPEEFNTLGNIARELGFSHVRSGPLVRSSYHAAE-------------

General information: TITO was launched using: RESULT: Template: 4U0O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1433 -20246 -14.13 -76.11 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -14.13 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.578

(partial model without unconserved sides chains): PDB file : Tito_4U0O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U0O-query.scw PDB file : Tito_Scwrl_4U0O.pdb: