Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKDPSDYTVTQESVLKLIQEQKRMNREMITELEQIHGPFPISHDIQYIKVLLDSSNTHIVQDLMSVSKQLYKKTL 2WAF Chain:A ((427-464)) ----------------------------------QAYGSFPITA----VQALEYSSNTYMVQTALGLMGQTYQPNM

General information: TITO was launched using: RESULT: Template: 2WAF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 52 -5593 -107.56 -147.18 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -107.56 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.322

(partial model without unconserved sides chains): PDB file : Tito_2WAF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WAF-query.scw PDB file : Tito_Scwrl_2WAF.pdb: