Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLFGMIFLIATVAFILLGVLLKL-------AAFFFVSILTLIAAIVLFTVLKKNQHNQT 4EV6 Chain:A ((286-337)) ----TTIFAVPMWITGIYGMNFSYLPLANNPQGFWLVMALMVVIIMIFVYIFRRSG----

General information: TITO was launched using: RESULT: Template: 4EV6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 74 -26426 -357.11 -587.24 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -357.11 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.746

(partial model without unconserved sides chains): PDB file : Tito_4EV6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EV6-query.scw PDB file : Tito_Scwrl_4EV6.pdb: