Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPYVTVKMLEGRTDEQKRNLVEKVTEAVKETTGASEEKIVVFIEEMRKDHYAVAGKRLSDME 2OP8 Chain:A ((1-61)) -PYVTVKMLEGRTDEQKRNLVEKVTEAVKETTGASEEKIVVFIEEMRKDHYAVAGKRLSDME

General information: TITO was launched using: RESULT: Template: 2OP8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 148 -15661 -105.82 -256.74 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.98 3D Compatibility (PKB) : -105.82 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.98 QMean score : 0.722

(partial model without unconserved sides chains): PDB file : Tito_2OP8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OP8-query.scw PDB file : Tito_Scwrl_2OP8.pdb: