Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKRTAFVMGASQGIGKAIALKLADQHFSLVINSRNLDNIESVKEDILAKHPEASVIVLAGDMSDQHTRAGIFQKIESQCGRLDVLINNIPGGAPDTFDNCNIEDMTATFTQKTVAYIDAIKRASSLMKQNEFGRIINIVGNLWKEPGANMFTNSMMNAALINASKNISIQLAPHNITVNCLNPGFIATDRYHQFVENVMKKNSISKQKAEEQIASGIPMKRVGSAEETAALAAFLASEEASYITGQQISADGGSMKSI 3U49 Chain:A ((2-259)) -SKRTAFVMGASQGIGKAIALKLADQHFSLVINSRNLDNIESVKEDILAKHPEASVIVLAGDMSDQHTRAGIFQKIESQCGRLDVLINNIPGGAPDTFDNCNIEDMTATFTQKTVAYIDAIKRASSLMKQNEFGRIINIVGNLWKEPGANMFTNSMMNAALINASKNISIQLAPHNITVNCLNPGFIATDRYHQFVENVMKKNSIS----------GIPMKRVGSAEETAALAAFLASEEASYITGQQISADGGSMKSI

General information: TITO was launched using: RESULT: Template: 3U49.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1356 -115473 -85.16 -465.62 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -85.16 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.619

(partial model without unconserved sides chains): PDB file : Tito_3U49.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3U49-query.scw PDB file : Tito_Scwrl_3U49.pdb: