Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKVLVTGAGGQLGLELCRQLKQAGYEVIALTKKMMNIADQRSVRHSFGHYQPDIVVNSAAFTSVDQCEKELDKAYLINGIGAYYTALESTRIGAQYVHISTDYVFNGKGTQPYREDDPLDPKTIYGKSKRLGEELIRLTTKDSTIIRTSWVYGHGGSNFVETMLKLAETKQELRVVSDQIGSPTYTKDLAEAVIKLFSHPP-GIYHVSNSGICSWYEFATAIMEESGLETAILSVTTEEYGNKTPRPAYSVLSHRAIE-EAGIRPRHWREALREYLQERSSACD 1VL0 Chain:A ((14-287)) --KILITGANGQLGREIQKQLKGKNVEVIPTDVQDLDITNVLAVNKFFNEKKPNVVINCAAHTAVDKCEEQYDLAYKINAIGPKNLAAAAYSVGAEIVQISTDYVFDGEAKEPITEFDEVNPQSAYGKTKLEGENFVKALNPKYYIVRTAWLYGD-GNNFVKTMINLGKTHDELKVVHDQVGTPTSTVDLARVVLKVIDEKNYGTFHCTCKGICSWYDFAVEIFRLTGIDVKVTPCTTEEFPRPAKRPKYSVLRNYMLELTTGDITREWKESLKEYI--------

General information: TITO was launched using: RESULT: Template: 1VL0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1594 -91448 -57.37 -336.21 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -57.37 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_1VL0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VL0-query.scw PDB file : Tito_Scwrl_1VL0.pdb: