Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMAKSYLITGGAGFIGLTFTKLMLRETDA-RITVLDKLTYASHPEEMEKLKQNSRFRFVKGDISVQEDIDRAFDE-TYDGVIHFAAESHVDRSISQAEPFITTNVMGTYRLAEAVLKGKAKKLIHISTDEVYGDLKADDPAFTETTPLSPNNPYSASKASSDLLVLSYVKTHKLPAIITRCSNNYGPYQHSEKMIPTIIRHAKQGLPVPLYGDGLQIRDWLFAEDHCRAIKLILEKGTDGEVYNIGGGNERTNKELASVILKHLG-CEELFAHVEDRKGHDRRYAINASKLKNELGWRQEVTFEEGIARTIQWYTDNDR
4EGB Chain:A ((24-338))-AMNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLNNVKSIQDHPNYYFVKGEIQNGELLEHVIKERDVQVIVNFAAES---------IPFYDTNVIGTVTLLELVKKYPHIKLVQVSTDEVYGSL-GKTGRFTEETPLAPNSPYSSSKASADMIALAYYKTYQLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCSAIDVVLHKGRVGEVYNIGGNNEKTNVEVVEQIITLLGKTKKDIEYVTD--GHDRRYAINAEKMKNEFDWEPKYTFEQGLQETVQWYEKNE-


General information:
TITO was launched using:
RESULT:

Template: 4EGB.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1714 24083 14.05 80.01
target 2D structure prediction score : 0.67
Monomeric hydrophicity matching model chain A : 0.85

3D Compatibility (PKB) : 14.05
2D Compatibility (Sec. Struct. Predict.) : 0.67
1D Compatibility (Hydrophobicity) : 0.85
QMean score : 0.536

(partial model without unconserved sides chains):
PDB file : Tito_4EGB.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-4EGB-query.scw
PDB file : Tito_Scwrl_4EGB.pdb: