Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGSADERRFEVLRAIVADFVATQEPIGSKSLVERHNLGVSSATVRNDMAVLEAEGYITQPHTSSGRVPTEKGYREFVDRLEDVKPLSSAERRAIQSFLESGV-DLDDVLRRAVRLLAQLTRQVAVVQYPTLSTSTVRHLEVIALTPARLLMVVITDSGRVDQRIVELGDVIDDHQLAQLREI---LGQALEGKKLSAASVAVADLASQLGGAGGLGDAVGRAATVLLESLVEHTEERLLLGGTANLTRNAADFGGSLRSILEALEEQVVVLRLLAAQQEAGKVTVRIGHETASEQMVGTSMVSTAYGTAHTVYGGMGVVGPTRMDYPGTIASVAAVALYIGDVLGAR 1STZ Chain:A ((14-304)) --KLNDRQRKVLYCIVREYIENKKPVSSQRVLEVSNIEFSSATIRNDMKKLEYLGYIYQPHTSAGRIPTDKGLRFYYEEMLKISKETSEADLAVETFKSMPLADPEKVLFLAGNLLARLTEGYVLIERPNTRDLKILRVMLIPVSEDYLIFSILTEFG------VSKVTPIKTQERLNWEEIERQLNFLLRGR--TVGEVLMGKIES-LKGSGFLR---------LIESLIGETVERYLDAGLENLLKDETLTLEDIRNLLEEVKDQKFLESLVGEG-----ITVRIGREIGRKKLEKFAVFSGKYFKGESPIGSV-------------------------------

General information: TITO was launched using: RESULT: Template: 1STZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -120358 for 1986 contacts (-60.6/contact) + 2D Compatibility (PS) -30885 + (NN) -10630 + (LL) 3832 1D Compatibility (HY) -24000 + (ID) 4450 Total energy: -186491.0 ( -93.90 by residue) QMean score : 0.349

(partial model without unconserved sides chains): PDB file : Tito_1STZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1STZ-query.scw PDB file : Tito_Scwrl_1STZ.pdb: