Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQPSENRLITSARYALCLLTASGVLLSGCASSGVGSVAQTTRAEYYPSCYEPVSHLRSTDNAVRNSAITGAITGGLLGGLAGGLASDENRGRNAALAAAGGALAGGAAGYYMEKQKQISDDRARIGSYGTDVDRSTVEINRSVAYAKSAQSCYQSQFKALLDGRKNKSINEAEGRKRLAEIVSGLQETNALLVAANGRAGENISNYTQAYEKDLQQVGVPRAEVTKVAEAENRASTTKGGSKPKTGSNPKVPKEAVATEQTIRKAQDAQSEGNKVASQGQGMIREVCN-SPDMGDWAPPSCAKA 4MKV Chain:A ((406-444)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AVANRVALEACVQARNEGRDLAREGNAIIREACKWSPELAAACEVWKEIK

General information: TITO was launched using: RESULT: Template: 4MKV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) 6332 for 204 contacts (31.0/contact) + 2D Compatibility (PS) -4069 + (NN) -1486 + (LL) 13356 1D Compatibility (HY) -2400 + (ID) 700 Total energy: 11033.0 ( 54.08 by residue) QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_4MKV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MKV-query.scw PDB file : Tito_Scwrl_4MKV.pdb: