Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHVLIAVILFFLSIGLSAGCAEAGNKTQNQSATEVNASPLQPAEYFIYHNLMND-----------KGLIKTDFSDQPSYLSESLGLWMEFLLSK-----NDAPHFQDQYQHLTDSFLM-SNHLVTWKIQNGQ---ASGTNALIDDMRIMLSLDQAAAKWGRS---DYAQTARDIG-TSLKTYNMNNGFFTDFYDSQA-ASKDVTLSYVMPDALAVLKKNG--IIDEETEQRNANVLYSAP---L-KNGFLPKTYSSET-----KEYTY---DSEINLIDQLYAAWHLPEG----DEK-----ASVLADWIKQEFQKN-GKLYGRYSAYTKEPAVQYESPSVYALAVLFLTKQHENSSVIKAIYDRMNDFEIHDPVKSYYGGYMSGTQTHSFDNLLPLLAERKLFNENIIQ 1V5D Chain:A ((24-377)) --------------------------------QESLNASVRSYYDNWKKKYLKNDLSSLPGGYYVKGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNLMGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKASNVTNNNQLNLGDWDSKSSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSNKYSPNTGLISDFVVKNPPQPAPKDFLDESEYTNAYYYNASRVPLRIVMDYAMYGEKRSKVISDKVSSWIQNKTNGNPSKIVDGYQ-LNGSNIGSYPTAVFVSPFIAASITS----SNNQKWVNSGWDWM-KNK------------RERYFSDSYNLLTMLFI-------

General information: TITO was launched using: RESULT: Template: 1V5D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1642 -92916 -56.59 -304.64 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -56.59 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_1V5D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V5D-query.scw PDB file : Tito_Scwrl_1V5D.pdb: