Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTSNRRMGLFYVITGATCWGIGGTVAKKLFQEYQIDVNWLVTTRLLTAGMLLLMLQLFRKDRSQVIEVWKHKASAGQLLIFGLLGMLAVQYTYMASIQHGNAAVATLLQYLSPVIVIIYTLLRKQTVLTKQDIITVVLALVGCFFLLTNGSMSQLSVPAAAVVWGVLSGFAAAFYTLYAVGLLNKFDSLVVVGWAMIIGGFALGFIHPPWQLDFQRLTAEAYAYILFVILFGTMIAFWFFIKSLESLSPKETSLLGSLEPLSAVVTTVVWLKAPFGSFQWIGAICIIGITLILALHKEPSMESEKSILKIRNHANRDI 2JP3 Chain:A ((23-40)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IFGGLLCIAGIALALSGK---------------------

General information: TITO was launched using: RESULT: Template: 2JP3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2 -377 -188.50 -20.94 target 2D structure prediction score : 0.89 Monomeric hydrophicity matching model chain A : 0.39 3D Compatibility (PKB) : -188.50 2D Compatibility (Sec. Struct. Predict.) : 0.89 1D Compatibility (Hydrophobicity) : 0.39 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_2JP3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JP3-query.scw PDB file : Tito_Scwrl_2JP3.pdb: