Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSDNNFVSETLENVQYLLPGAKVIKLRGYSRAHKV-YTIAKSPVEKWKVAAGLSGSEIA-ILIRKGHWIGASIPAGGIVIDIDDSKQGELVKGLLDAQNFHCHCIRTLMGGSLFLRITNMGKRKLNK 3A21 Chain:A ((6-71)) ---------------QITVPSAPMGWASWNSFAAKIDYSVIKKQVDAF-VAAGLPAAGYTYINIDEGWWQGTRDSAGNITVD----------------------------------------------

General information: TITO was launched using: RESULT: Template: 3A21.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 127 -15956 -125.63 -249.30 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -125.63 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.172

(partial model without unconserved sides chains): PDB file : Tito_3A21.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A21-query.scw PDB file : Tito_Scwrl_3A21.pdb: