Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFFRNQLANVVEWEEFRDDMIFYKWNNREIKKGSRLIIRPGQDAVFLNNGRVEGVFQDDGDYDIESEIIPFLSTLKGFKFGFNSGMRAEVLFVNTKEFTVRWGTKQAINIPAAGMPGGMP----IRANGTFNFK-VQDYISLIDKIAGVKDQYFVEDIKT-RIISILDQLLMKWITREGKDMFNLQANAFDIAKGIQEDLDMQLISDGMTVTGFQIMSFNYPQEVQDMITKNASYGMVGDVNRYQQISMTDGMASGKMSGSGAASDMAGMMMGMNMANQMMNQMNQNQQAQSSGPQSTGSGSKPNFCPNCGTKTGEANFCPNCGQKLV 5FWY Chain:B ((92-260)) -----------------------------------------------LHTSFVTPSFPTDADVQFVIQMRP---ALKGAILSLLSYYKWE-------KFVYLYDTERGFSVLQAIMEAAVQNNWQVTARSVGNIKDVQEFRRIIEEMDRRQEKRYLIDCEVERINTILEQVVI--LGKHSRGYHYMLANL-----GFTDILLERVMHGGANITGFQIVNNENPM-VQQFIQR-------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FWY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 614 -70539 -114.88 -438.13 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -114.88 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.117

(partial model without unconserved sides chains): PDB file : Tito_5FWY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FWY-query.scw PDB file : Tito_Scwrl_5FWY.pdb: