Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVIQVKGNVTYPITIDPSVWIFDDRKFSFDRKGDSQDTYLQSAGDESDLDPERVIREGRIAPPTLKTEKQYEKQKLMNGSFAMRLGTVLKNAEPNSSATQCVFVTSS-----G-KAAVSLETAL----NSIVHFSEAGKPIQ--EGGPVHIYFEDPVHHKQPITDVKEIEII 2BII Chain:A ((109-199)) ---------------------------------------------------------------------------------TGCMLGDVIGKARPSKRARFVWMEGADNPANGAYGTCIRLSWCMDPERCIMIAYQQNGEWLHPDHGKPLRVVIPGVIGGRS-VKWLKKLVVS

General information: TITO was launched using: RESULT: Template: 2BII.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 342 -66586 -194.70 -842.86 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -194.70 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.705

(partial model without unconserved sides chains): PDB file : Tito_2BII.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BII-query.scw PDB file : Tito_Scwrl_2BII.pdb: