Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVPKTMLLSTAAGLLLSLTATSVSAHYVNEEHHFKVTAHTETDPVASGDDAADDPAIWVHEKHPEKSKLITTNKKSGLVVYDLD-GKQLHSYEF-GKLNNVDLRYDFPLNGEKIDIAAASNRSEGKNTIEVYAIDGDKGKLKSITDPNHPISTNISEVYGFSLYHSQKTGAFYALVTG-KQGEFEQYEIVDGGKGYVTGKKVREFKLNSQTEGLVADDEYGNLYIAEEDEAIWKFNAEPGGGSKGQVVDRATGDHL------TADIEGLTIYYAPNGKGYLMASSQGNNSYAMYERQGKNRYVANFEITDGEKIDGTSDTDGIDVLGFGLGPKYPYGIFVAQDGENIDNGQAVNQNFKIVSWEQIAQHLGEMPDLHKQVNPRKLKDRSDG 3U4Y Chain:A ((109-304)) -----------------------------------------------------------------------------NMQSYSFLKNKFISTIPIPYDAVGIAISPN------GNGLILIDRSSA--NTVRRFKIDADG-VLFDTGQ---EFISGGTRPFNITFTP---DGN-FAFVANLIGNSIGILETQNPE--N--ITLLNAVGTNNLPGTIVVSRDGSTVYVLTE-STVDVFNFNQLSGT-LSFVK-SFGHGLLIDPRPLFGANQMALN--KTE-TKLFISANISRELKVFTISG--------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3U4Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1007 23752 23.59 127.01 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 23.59 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_3U4Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3U4Y-query.scw PDB file : Tito_Scwrl_3U4Y.pdb: