Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIQDMILTLQKHWSSQGCVLMQAYDVEK---------GAGTMSPY--TFLRSIGPEPWKVAYVEPSRRP----ADG-RYGENPNRLYQHH---------------------QFQVIIKP--SPDNIQELYLD-------SLR----ALGIDPLEHDIRF--------VEDNWENP-SLGCAGLGWEVWLD-------------------GMEITQFTYFQQ---V-GG-LECKPVSVEITYGIERLASYIQDKENVFDLEWTSGFTVKDLFMMAEYEHSVYTFETSDVDMLFQLFSTYEKEAIKQMDNGLVHPAYDYVLKCSHTFNLLDAKGAISVTERTGYIARVRNLARKVAKTYYEEREKLGFPMLKGEGSSHE 3HY0 Chain:A ((2-256)) KSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAG-MNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTA---RHHTFFEMLGNFSFGDYFKLDAILFAWLLLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGI-PRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTAMGLERIAAVLQHVNSNYDI----------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3HY0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 647 3037 4.69 17.65 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 4.69 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.314

(partial model without unconserved sides chains): PDB file : Tito_3HY0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HY0-query.scw PDB file : Tito_Scwrl_3HY0.pdb: