Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYDHILIRFGEISTKG-KNRKSFIERLKQNIRLVLKDYPNL---KYFSNRDRMTITLNGEDPEALFPHLKQVFGIQSFSLAIKCDSRLDDIKATALKAIKDQY----KPGDTFKVATKRAYKQFELDTNQMNAEIGGHILRNTEGLTVDVRNPDIPLRIEIREEATFLTIRDEKGAGGLPVGSAGKAMLMLSGGFDSPVAGFYAMKRGLSVEAVHFFSPPYTSERAKQKVMDLAKCLSRFGGSMTLHIVPFTKTQELIQKQIPENYTMTATRRLMLQIADRIREKRNGLAIITGESLGQVASQTLESMYAINAVTSTPILRPLIAMDKTEIIEKSREIGTYETSIQPFEDCCTIFTPPSPKTRPKKEKIEHFESFVDFEPYIQEAVDNIETMTLYSEQEANDKFAELF 1VBK Chain:A ((1-307)) --MNVVIVRYGEIGTKSRQTRSWFEKILMNNIREALVTE-EVPYKEIFSRHGRIIVKTNS--PKEAANVLVRVFGIVSISPAMEVEASLEKINRTALLMFRKKAKEVGKERPKFRVTARRITKEFPLDSLEIQAKVGEYILNNE-NCEVDLKNYDIEIGIEIMQGKAYIYTEKIKGWGGLPIGTEGRMIGILHDE-LSALAIFLMMKRGVEVIPVYIGKD----DKNLEKVRSLWNLL-----KRYSY--GSKGF------------LVVA---ESFDRVLKLIRDFGVKGVIKGLRPNDLNSEVSE-ITEDFKMFPVPVYYPLIALPEEYIKSVKERLGL--------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1VBK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1507 18248 12.11 61.03 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 12.11 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_1VBK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VBK-query.scw PDB file : Tito_Scwrl_1VBK.pdb: