Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFMFEKPHGMRDTLPGLYETKKKVRRSLTDLI----DKWGYQFMETPTLEFYDTVGVQSAIEE--QQLFKLLDQDGKTLVLRPDMTGPIARVAASKLLKHGHPLRVGYAANVFRAQEREGGRPAEFEQVGVELIGDGTTSADAEVIALVVGALKNAGLAS------------FKIAIGHAGIADALFVEVLGNVERADVLRRFLYEKNYVGYREHVKSLPLSSIDKSRLLELLELRGGIEVCGRAEEIVDSAQGKSVVDELKALWDILEDYGCTENVRLDLNMVSHMSYYTGILFEVYAENVGF-VIGSGGRYNKLLGHFDSPA--PATGFGLRIDRLIEALHMK----DEPCEIDAVI--FSKEQRAQAIAYANEERMKGNKVVLQDLSGIENIDQMTKSFANVTYFIGARKEEQNG 4YP0 Chain:A ((26-401)) MVETEPVQGCRDFPP----EAMRCRRHLFDVFHATAKTFGFEEYDAPVLE-SEELYIRKAGEEITEQMFNFITKGGHRVALRPEMTPSLARLLLGKGRSLLLPAKWYSIPQCWRYE-----RRREHYQWNMDIVGVKSVSAEVELVCAACWAMRSLGLSSKDVGIKVNSRKVLQTVVEQAGVTSDKFAPVCVIVDKMEKIPREEVEAQLA-----VLGLEPTVVDA--ITTTLSLKSIDEIAQRVGE------EHEAVKELRQFFEQVEAYGYGDWVLFDASVVRGLAYYTGIVFEGFDREGKFRALCGGGRYDNLLTTYGSPTPIPCAGFGFGDCVIVELLQEKRLLPDIPHVVDDVVIPFDESMRPHALAVLRRLRDAGR------------------------------------

General information: TITO was launched using: RESULT: Template: 4YP0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1680 -8747 -5.21 -26.34 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -5.21 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_4YP0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YP0-query.scw PDB file : Tito_Scwrl_4YP0.pdb: