Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGELANYKRLEKVGEGTYGVVYKALDLRPGQGQRVVALKKIRLESEDEGVPSTAIREISLLKELKDDNIVRLYDIVHSDAHKLYLVFEFLDLDLKRYME-----GIPKDQPLGADIVKKFMMQLCKGIAYCHSHRILHRDLKPQNLLINKDGNLKLGDFGLARAFGVPLRAYTHEIVTLWYRAPEVLLGGKQYSTGVDTWSIGCIFAEMCNRKPIFSGDSEIDQIFKIFRVLGTPNEAIWPDIVYLPDFKPSFPQWRRKDLSQVVPSLDPRGIDLLDKLLAYDPINRISARRAAIHPYFQES 4EOS Chain:A ((2-291)) --GSMENFQKVEKIGEGTYGVVYKARNKLTGE---VVALKKIRLD---EGVPSTAIREISLLKELNHPNIVKLLDVIHTE-NKLYLVFEFLHQDLKKFMDASALTGIP------LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQD-

General information: TITO was launched using: RESULT: Template: 4EOS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -183236 for 2215 contacts (-82.7/contact) + 2D Compatibility (PS) -29485 + (NN) -8568 + (LL) 316 1D Compatibility (HY) -38000 + (ID) 9250 Total energy: -268223.0 ( -121.09 by residue) QMean score : 0.507

(partial model without unconserved sides chains): PDB file : Tito_4EOS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EOS-query.scw PDB file : Tito_Scwrl_4EOS.pdb: