Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIQRETSGHIAAIVTILIWGTTFISTKVLLADFSPMEILFYRFLMGFIALILVRPNMIPFRNWRQELLFAGAGLFGVTLYFLLENIALTYTYASNVGMIVSIIPMITAVLAHFLLEGEKLRLTFLIGFISALIGLLLITFNGNVVLRLNPLGDIMAAGAALVFGGYSIFMKKLSAYEYHIIELTQRVFLYGLLFMVPALFLFDFHFDLSRFSSASNILNMLFLGIGASALCFATWNYSVGVLGAVKSSAYIYMVPVITIAASVLILHENMTWIALLGGAL--TLLGLYISELKPKAKLLENGCKMDA 3RKO Chain:J ((134-165)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FGPYVLAVELASMLLLAGLVVAFHVGREERAG----------

General information: TITO was launched using: RESULT: Template: 3RKO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 8 -1843 -230.31 -61.42 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain J : 0.36 3D Compatibility (PKB) : -230.31 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.36 QMean score : 0.141

(partial model without unconserved sides chains): PDB file : Tito_3RKO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RKO-query.scw PDB file : Tito_Scwrl_3RKO.pdb: