Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQNETRTLQLDPHLHIEAYRFKGIMQKFPNHFHDYYVIGFIEKGQRYLACQDQEYIINPGDLLLFNPRDTHSCEQIDGRTLDYRCINVMPDIMEKAVKEITGSGHLPYFSQHVLFRHELTASLQELHILISEEKQALRKEELFLHLLEELIRHYSDVTFLSSVPEPSDEVKMVCEFLEEHYAEN-VTLNDLSELTGWSKYHLLRSFTKQKGITPNSYMETIRINQAKKLLEQGVRP-IDAAFQTGFSDQSHMTKFFKRQVGLTPKQYMKIFEKELHR 3LSG Chain:A ((1-101)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------SNAKELIQNIIEESYTDSQFTLSVLSEKLDLSSGYLSIMFKKNFGIPFQDYLLQKRMEKAKLLLLTTELKNYEIAEQVGFEDVNYFITKFKKYYQITPKQY----------

General information: TITO was launched using: RESULT: Template: 3LSG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 319 -35025 -109.79 -353.78 target 2D structure prediction score : 0.87 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -109.79 2D Compatibility (Sec. Struct. Predict.) : 0.87 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.599

(partial model without unconserved sides chains): PDB file : Tito_3LSG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LSG-query.scw PDB file : Tito_Scwrl_3LSG.pdb: