Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQYASESINLPGEITFKDVREIFFYQIAKISCFYFLLFCAIFAAVNFINGWPRIVYGSDALNLFMNSMLIIVMSVLFTLLLLLLLYVKFSRAY---KKNERMKSKRTYTLNQEGIRICSKKYDLIFNWDEIT-----AVFEYKN----IFRVNTSSGQYIAIPKHFFHSEE----EMN-RFKEIILKNTETKKLKFKKDQH 2Z8L Chain:A ((55-165)) ----------------------------------------------------------------------------------------KFTRIQIFGKDIERLKTRKNPGLDIFVVKEAENRNGTVFSYGGVTKKNQGAYYDYLNAPKFVIKKEVDAGVYTHVKRHYIYKEEVSLKELDFKLRQYLIQNFDLYK-KFPKD--

General information: TITO was launched using: RESULT: Template: 2Z8L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 205 -2414 -11.77 -25.68 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -11.77 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_2Z8L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z8L-query.scw PDB file : Tito_Scwrl_2Z8L.pdb: