Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTH-VEIDAKRYGFERDSVILLEQIRTID-KQRLTDKITHLDDEMMDKVDEALQISLALIDF 1M1F Chain:A ((1-101)) --MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVPVTS---FARTAGFAVSLDG--VGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEV-------------

General information: TITO was launched using: RESULT: Template: 1M1F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 422 -53292 -126.28 -555.12 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -126.28 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.370

(partial model without unconserved sides chains): PDB file : Tito_1M1F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M1F-query.scw PDB file : Tito_Scwrl_1M1F.pdb: