Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMSNQTVYQFIAENQNELLQLWTDTLKELSEQESYQLTDQVYENISKEYIDILLLSVKDENAAESQISELALRAVQIGLSMKFLATALAEFWKRLYTKMNDKRLPDQESTELIWQIDRFFSPINTEIFNQYSISWEKTVSLQKIALQELSAPLIPVFENITVMPLVGTIDTERAKRIMENLLNGVVKHRSQVVLIDITGVPVVDTMVAHHIIQASEAVRLVGAKCLLAGIRPEIAQTIVNLGIDLSQVITKNTLQKGIQTALEMTDRKIVSLGE 3ZTB Chain:B ((32-111)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EELLHNITGVAGKGLVIDISALEVVDSFVTRVLIEISRLAELLGLPFVLTGIKPAVAITLTEMGLDLRGMATALNLQKGL----------------

General information: TITO was launched using: RESULT: Template: 3ZTB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 268 -75303 -280.98 -941.28 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : -280.98 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.618

(partial model without unconserved sides chains): PDB file : Tito_3ZTB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZTB-query.scw PDB file : Tito_Scwrl_3ZTB.pdb: