Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFEKIRKWLEITNEYKQSDFWTNVLKYKAPEHFFDSEASTFVYDFYQDEEYNFIIVEMPGVYEEELTIRLLSKTQLLIKGTITPVFPAEMEVLRERYYGEIE------RIIQLPEAAETHLLQIQLLNGLLHISYPRQVETVAFNKGL 4ELD Chain:A ((70-158)) ------------------------------------------------------VIAWLPGVNKEDIILNAVGDT-LEIRAKRSPLMITESERI---IYSEIPEEEEIYRTIKLPATVKEENASAKFENGVLSVILPKAESSI--KKGI

General information: TITO was launched using: RESULT: Template: 4ELD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 258 -42718 -165.57 -514.67 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -165.57 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.350

(partial model without unconserved sides chains): PDB file : Tito_4ELD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ELD-query.scw PDB file : Tito_Scwrl_4ELD.pdb: