Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKQGNQMSFLRTIILVSTFGGLLFGYDTGVLNGALPYMGE------PDQLNLNAFTEGLVTSSLLFGAALGAVFGGRMSDFN--------GRRKNILFLAVIFFISTIG------CTFAPNVTVMIISRFVLGIAVGGASVTVPAYLAEMSPVESRGRMVTQNELMIVSGQLLAFVFNA--ILGTTMGDNSHVWRFMLVIASLPALFLFFGMIRMPESPRWL-VSKGRKEDALRVLKKIRDEKRAAAELQEIEFAFKKEDQLEKATFKDL-SVPWVRRIVFIGLGIAIVQQITGVNSIMYYGTEILRNSGFQTEAALIGNIANGVISVLATFVGIWLLGRVGRRPMLMTGLIGTTTALLLIGIFSLVLEGSPALPYVVLSLTVTFLAFQQGAISPVTWLMLSEIFPLRLRGLGMGVTVFCLWMVNFAVSFTFPILLAAIGLSTTFFIFVGLGICSVLFVKRFLPETKGLSLEQLEENFRAYDHSGAKKDSGAEVIG 4ZWC Chain:A ((36-492)) --------------ITVATIGSFQFGYNTGVINAPEKIIKEFITKTLTDKGNAPP-SEVLLTSLWSLSVAIFSV-GGMIGSFSVGLFVNRFGRRNSML---IVNLLAVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILGS-----EELWPLLLGFTILPAILQSAALPFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKDAGVQ--EPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMTVSLLLKDNYNGMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAHYLG-AYVFIIFTGFLITFLAFTFFKVPETRGRTFEDITRAFEGQAH-------------

General information: TITO was launched using: RESULT: Template: 4ZWC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2155 -288711 -133.97 -666.77 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -133.97 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_4ZWC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZWC-query.scw PDB file : Tito_Scwrl_4ZWC.pdb: