Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFWKNKNVFVTGCTGLLGSYLVKELIEQGANVTGLVRDHVPQSNLYQGEHIK---KMNIVRGSLEDLAVIERALGEYEIDTVFHLAAQAIVGVANRNPISTFEANILGTWNILEACRKHPLIK--------RVIVASSDKAYGDQEN---------LP-YDENMPLQGKHPYDVSKSCADLISHTYFHTYGLPVCITRCGNLYGGGDLNFNRIIPQTIQLVLNGEAPEIRSDGTFVRDYFYIEDAVQAYLLLAEKMEENNLAGEAFNFSN-------EIQLTVLELVEKILKKMNSNLK--PKVLNQGSNEIKHQYLSAEKARKLLNWTPAYTIDEGLEKTIEWYTEFFKK 1G1A Chain:A ((3-334)) --------ILITGGAGFIGSAVVRHIIKNTQDTVVNIDKLTYAGNLESLSDISESNRYNFEHADICDSAEITRIFEQYQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYALLEVARKYWSALGEDKKNNFRFHHISTDEVYGDLPHPDEVENSVTLPLFTETTAYAPSSPYSASKASSDHLVRAWRRTYGLPTIVTNCSNNYGPYHFP-EKLIPLVILNALEGKPLPIYGKGDQIRDWLYVEDHARALHMVVTEGK----AGETYNIGGHNEKKNLDVVFTICDLLDEIVPKATSYREQITYVADRPGHDRRYA-IDAGKISRELGWKPLETFESGIRKTVEWY------

General information: TITO was launched using: RESULT: Template: 1G1A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1626 -144618 -88.94 -478.87 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -88.94 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_1G1A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G1A-query.scw PDB file : Tito_Scwrl_1G1A.pdb: