Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYHYFYGPALIPYFSHTHNPQRSPDFSERQRRREITLTVDGF---VPPSSTEDLGDGFYSYAWTNQQVINPGEYLVCHLEGRDINVINGGFTPRDTAPLYAVASFPRRRNQWVIIIHNPVQTQRAISLYLIAKR 3FFE Chain:A ((183-246)) --------------AHLGQEQWAPEFQARAALHQFEVPVDGLHIGANGLTPQQVLDGF-----ADQQPASPGHAIIC---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3FFE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 119 -11801 -99.16 -214.55 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -99.16 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.332

(partial model without unconserved sides chains): PDB file : Tito_3FFE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FFE-query.scw PDB file : Tito_Scwrl_3FFE.pdb: