Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAQKTFKVTADSGIHARPATVLVQTASKYDADVNLEYNGKTVNLKSIMGVMSLGIAKGAEITISASGADENDALNALEETMKSEGLGE 1JEM Chain:A ((2-88)) -AQKTFKVTADSGI-ARPATVLVQTASKYDADVNLEYNGKTVNLKSIMGVVSLGIAKGAEITISASGADENDALNALEETMKSEGLGE

General information: TITO was launched using: RESULT: Template: 1JEM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 399 -49330 -123.63 -573.60 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -123.63 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.628

(partial model without unconserved sides chains): PDB file : Tito_1JEM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JEM-query.scw PDB file : Tito_Scwrl_1JEM.pdb: