Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHSVVELIEIESAIIQVKMQDRTHKNAFSQELTDDLIQAFEYIRQNPKYKAVILTGYDNYFASGGTQEGLL------------RIQQGLTKFTDDNLYSLALDCEIPVIAAMQGHGIGGGFVMGLFADIVILSRESVYTANFMKYGFTPGMGATFIVPKKLGFSLAQEILLNAGSYRGADLEKRGVPFKVLPRAEVLDYAVELAQELAEKPRNSLVTLKDHLVAPLRDQLPRVIEQELMMHEKTFHHEEVKSRIKGLYGN 4JCS Chain:A ((7-249)) ---------EVVNNVGWIRMNRAAKHNPFDAELRADLMTVLERVRDDADIRVLVLTSHPGSFCA--NLHVLRDNLDSGPAYWQQRIKTGL-RFIHD-----MLNLGRPVIAAVDGPAFGAGFALSLTADIVLASPRARFSMAYLRLGLVPDLGALYLLPRAVGLQRAKELMFSTRELDAEEAHRLGLVMEVHESEALEQRAREIAESLVQAAPTALALTKAALNVSLDSDEQTMFSLEAASQAAAFSTKEPRAAIEAL---

General information: TITO was launched using: RESULT: Template: 4JCS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1058 -144492 -136.57 -630.97 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -136.57 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.557

(partial model without unconserved sides chains): PDB file : Tito_4JCS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JCS-query.scw PDB file : Tito_Scwrl_4JCS.pdb: